Species-specific diagnostics of Apis mellifera trypanosomatids: nine-year survey (2007-2015) Serbia

Abstract In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were…

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Antiproliferative activity & apoptotic effects of Filipendula ulmaria against C26...

Abstract Honeybee collected pollen exhibits high nutritional and pharmaceutical benefits for the human diet and medicine. Pollen’s antioxidant, anti-ageing, anti-inflammatory, anti-atherosclerosis, and cardioprotective activity, depending on the floral origin, are well known. Recent studies proposed that pollen may also be an excellent cancer-fighting candidate, as pollen harbours high amounts of phenolic substances. In our study,…

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Effects of treatments with Apivar & Thymovar on V. destructor...

Abstract Efficacies of two miticides, Apivar and Thymovar, were evaluated as a fall treatment against V. destructor. The effect of treatment with miticides was further evaluated by monitoring both viral load and rate of indoor overwintering survival of colonies of European honey bees (Apis mellifera L.) in the vicinity of Saskatoon, Saskatchewan, Canada. Forty-five colonies…

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Bay laurel (Laurus nobilis) as potential antiviral treatment in naturally...

Abstract Viral diseases are one of the multiple factors associated with honeybee colony losses. Apart from their innate immune system, including the RNAi machinery, honeybees can use secondary plant metabolites to reduce or fully cure pathogen infections. Here, we tested the antiviral potential of Laurus nobilis leaf ethanolic extracts on forager honeybees naturally infected with BQCV (Black…

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Comparison of tau-fluvalinate, acrinathrin & amitraz effects on susceptible popul....

Abstract The parasitic mite Varroa destructor is a major pest of the western honeybee, Apis mellifera. The development of acaricide resistance in Varroa populations is a global issue. Discriminating concentrations of acaricides are widely used to detect pest resistance. Two methods, using either glass vials or paraffin capsules, are used to screen for Varroaresistance to various acaricides. We found the glass vial…

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Multi-residue method for the determination of pesticides and pesticide metabolites...

Abstract A method for the determination of 200 pesticides and pesticide metabolites in honeybee samples has been developed and validated. Almost 98% of compounds included in this method are approved to use within European Union, as active substances of plant protection products or veterinary medicinal products used by beekeepers to control mites Varroa destructor in…

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Global honey trade but not pests or pesticides as a...

Abstract Recent losses of honeybee (Apis mellifera) colonies have been linked to several non-exclusive factors; such as pests, parasites, pesticides (e.g., neonicotinoids) and other toxins. Whereas these losses pose a threat to apiculture, the number of globally managed colonies appeared to be less affected because beekeepers replace lost colonies. From a socioeconomic and ecological perspective…

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Four Categories of Viral Infection Describe the Health Status of...

Abstract Honey bee virus prevalence data are an essential prerequisite for managing epidemic events in a population. A survey study was carried out for seven viruses in colonies representing a healthy Danish honey bee population. In addition, colonies from apiaries with high level Varroainfestation or high level of winter mortality were also surveyed. Results from RT-qPCR…

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Effects of genotype, environment, and their interactions on honey bee...

Abstract There are several reports of honey bee populations in Europe which survive without treatment for Varroa. However, when evaluated outside their native area, higher survival and resistance traits were not observed in colonies of a survivor population. Varroainfestation is strongly influenced by environmental factors, probably affecting threshold levels on a European scale. In a Europe-wide experiment…

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Effects of Environmentally-Relevant Mixtures of 4 Common Organophosphorus Insecticides on...

Abstract We assessed whether exposure to environmentally-relevant mixtures of four organophosphorus insecticides (OPs) exerted adverse effects on honey bees. Adult and worker bees were orally exposed for five days under laboratory conditions to mixtures of four insecticides, diazinon, malathion, profenofos and chlorpyrifos at two concentrations. Concentration in the mixtures tested were equivalent to the median…

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