In vitro study on enzymatic systems of honeybees

In this study we measured in vitro honey bee enzyme activities in presence of the synergist piperonyl butoxide (PBO) and some new benzodioxole and dihydrobenzofuran derivatives through absorbance and fluorescence spectrophotometric assays, to determine if and to what extent detoxification systems and acetylcholinesterases are affected by these compounds. Both Italian (Italy) and Carniolan (Czech Republic)…

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Origin of antimicrobial properties of propolis

Abstract   Natural honey bee products, especially propolis are well known for their biological properties. However, less well known are the impact of propolis’ single phenolic substances and variation across different landscapes, geographic locations and botanical origins. Here, we determined the chemical composition of propolis samples collected in different geographic areas of Romania with a…

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Microsporidian Nosema neumanni

Abstract The microsporidium Nosema neumanni n. sp., a new parasite of the honeybee Apis mellifera is described based on its ultra-structural and molecular characteristics. Structures resembling microsporidian spores were found by microscopic examination of honeybees from Uganda. Molecular confirmation failed when PCR primers specific for Nosema apis and Nosema ceranae were used, but was successful…

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Trueness and precision of the real-time RT-PCR method

Abstract The Chronic bee paralysis virus (CBPV) is the aetiological agent of chronic bee paralysis, a contagious disease associated with nervous disorders in adult honeybees leading to massive mortalities in front of the hives. Some of the clinical signs frequently reported, such as trembling, may be confused with intoxication syndromes. Therefore, laboratory diagnosis using real-time PCR to…

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Achromobacter eurydice, a Gram-variable pleomorphic bacterium associated with European foulbrood

Abstract Honeybees are prone to parasite and pathogen infestations/infections due to their social colony life. Bacterial pathogens in particular lead to destructive infections of the brood. European foulbrood is caused by the bacterium Melissococcus plutonius in combination with several other Gram-positive bacteria (Achromobacter eurydice, Bacillus pumilus, Brevibacillus laterosporus, Enterococcus faecalis, Paenibacillus alvei, Paenibacillus dendritiformis) involved as secondary invaders following the initial infection.…

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